TOAST 4.5 AstroBotany

TOAST: Test Of Arabidopsis Space Transcriptome

The TOAST database: Detailed tutorials and experimental protocols found @ www.astrobotany.com

1. The first tab provides an video introducing the NASA GeneLab program.

2. The second video describing how to send an experiment to space.

3. The GeneLab metadata exploration tab then allows you to explore and filter the meta-data associated with GeneLab AstroBotany experiments.

4.  Activate significance threshold filters to select groups of genes and observe the Genome Ontology (GO) Biological processes, GO cellular site, GO molecular function or KEGG biochemical pathways they are involved with.

Genetic Network Analysis

Network showing bioinformatic links realted to Calcium signaling

Above: Example Network diagram produced showing links between spaceflight response genes based on the NCBI literature, protein sequence, co-expression and functional prediction made using gene identified in TOAST and visualized using Knetminer.